Journal: Biomolecules
Article Title: Postmortem Stability Analysis of Lipids and Polar Metabolites in Human, Rat, and Mouse Brains
doi: 10.3390/biom15091288
Figure Lengend Snippet: Experiment outline. ( A ). Schematic representation of experimental setup. ( B ). Numbers of detected lipid compounds, grouped according to their biochemical properties. Lipid class abbreviations stand for: AHexCer—acylhexosylceramide; BMP—bis(monoacylglycero)phosphate; CAR—acylcarnitine; CE—cholesteryl ester; Cer—ceramide; CL—cardiolipin; CoQ—coenzyme Q; DG—diacylglycerol; FA—fatty acid; GM—ganglioside; HBMP—hydroxy-bis(monoacylglycero)phosphate; HexCer—hexosylceramide; LPC—lysophosphatidylcholine; LPC-O—lysophosphatidylcholine ether; LPE—lysophosphatidylethanolamine; LPE-O—lysophosphatidylethanolamine ether; LPI—lysophosphatidylinositol; LPS—lysophosphatidylserine; MGDG—monogalactosyldiacylglycerol; NAE—N-acylethanolamine; OxFA—oxidized fatty acid; PC—phosphatidylcholine; PC-O—ether-linked phosphatidylcholine; PE—phosphatidylethanolamine; PE-O—ether-linked phosphatidylethanolamine; PE-P—plasmalogen phosphatidylethanolamine; PG—phosphatidylglycerol; PI—phosphatidylinositol; PMeOH—phosphatidylmethanol; PS—phosphatidylserine; PS-O—ether-linked phosphatidylserine; SHexCer—sulfated hexosylceramide; SM—sphingomyelin; ST—sulfatide; TG—triacylglycerol; TG-O—ether-linked triacylglycerol. A list of the detected lipids is provided in . ( C ). Numbers of detected polar metabolite compounds, grouped according to their biochemical properties. A list of the detected polar metabolites is provided in .
Article Snippet: The extraction buffer (MeOH (Carlo reagents, CAS 67-56-1):MTBE (Sharlab, CAS 1634-04-4), (1:3 ( v / v )) was enriched with internal standards including 0.5 μg/mL triacylglycerol (TAG 15:0/18:1-d7/15:0, Avanti Polar Lipids, 791648C), diacylglycerol (DAG 15:0/18:1-d7, Avanti Polar Lipids, 791647C), ceramide (Cer d18:1-d7/15:0, Avanti Polar Lipids, 860681), lysophosphatidylcholine (LPC 18:1-d7, Avanti Polar Lipids, 791643C), phosphatidylglycerol (PG 15:0/18:1-d7, Avanti Polar Lipids, 791640C), phosphatidylcholine (PC 15:0/18:1-d7, Avanti Polar Lipids, 791637C), phosphatidylethanolamine (PE 15:0/18:1-d7, Avanti Lipids, 791638C), methionine-methyl-13C, d3 (Aldrich 299154), and D-L-Aspartic-acid-4 C13 (Aldrich 488968).
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